| 產品編號 | bs-0498R |
| 英文名稱 | ADRB1 Rabbit pAb |
| 中文名稱 | 腎上腺素能受體β1抗體 |
| 別 名 | beta 1 Adrenergic Receptor; beta 1 Adrenergic Receptor; ADRB1R; Adrenergic beta 1 receptor; B1AR; beta 1 adrenoceptor; beta 1 adrenoreceptor; beta-1 adrenergic receptor; beta-1 adrenoceptor; beta-1 adrenoreceptor; BETA1AR; RHR; ADR B1; ADRB 1; ADRB1; ADRB |
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Specific References (8) | bs-0498R has been referenced in 8 publications.
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| 研究領域 | 心血管 免疫學 神經生物學 信號轉導 生長因子和激素 通道蛋白 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 52 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADRB1: 181-250/477 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Beta-adrenergic receptors (Beta-1 adrenoreceptor) mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Subcellular location cell membrane; Multi-pass membrane protein. Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. Belongs to the G-protein coupled receptor 1 family. Function: Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Subunit: Interacts with GOPC, MAGI3 and DLG4. Subcellular Location: Cell membrane; Multi-pass membrane protein. Note=Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression. Post-translational modifications: Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. Similarity: Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily. SWISS: P08588 Gene ID: 153 Database links: Entrez Gene: 153 Human Entrez Gene: 11554 Mouse Omim: 109630 Human SwissProt: P08588 Human SwissProt: P34971 Mouse Unigene: 99913 Human Unigene: 46797 Mouse Unigene: 87064 Rat β1腎上腺素能受體（Beta-adrenergic Receptor-1 ）在接受交感神經節后纖維支配的各種器官中存在著與腎上腺素、去甲腎上腺素起反應的受體，稱為腎上腺素能受體。其化學性質尚不清楚。用對藥物反應的方法，腎上腺素能受體可分為α及β兩個類型。腎上腺素對α、β及D型受體均起作用，而去甲腎上腺素主要對α型起作用。 β1AR所引起的反應為支氣管擴張、血管擴張等，腎上腺素受體可被β13，4－dichloroisoproterenol（D－CI）等阻斷藥物所抑制。 |
| 產品圖片 |
Sample:
Heart (Mouse) Lysate at 30 ug
Heart (Rat) Lysate at 30 ug
Primary: Anti- ADRB1(bs-0498R) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution
Predicted band size: 52 kD
Observed band size: 55 kD
Tissue/cell: skin of rat foot; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(bs-0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(bs-0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:U937.
Primary Antibody (green line): Rabbit Anti- antibody (bs-0498R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-ADRB1 antibody (bs-0498R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: mouse kidney cell;
Incubation:
Red line: Anti-ADRB1 Polyclonal Antibody, FITC conjugated(bs-0498R-FITC) 1:200, 40 minutes at 37°C.
Green line: Isotype Control(bs-0295P-FITC, rabbit IgG/FITC) 1:200, 40 minutes at 37°C.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
