| 產(chǎn)品編號(hào) | bs-0489R |
| 英文名稱 | BrdU(Proliferation Marker) Rabbit pAb |
| 中文名稱 | 5-溴脫氧尿嘧啶核苷（增殖標(biāo)志物）抗體 |
| 別 名 | Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine. |
 |
Specific References (8) | bs-0489R has been referenced in 8 publications.
|
| 產(chǎn)品類型 | 小分子抗體 |
| 研究領(lǐng)域 | 細(xì)胞生物 細(xì)胞類型標(biāo)志物 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Species independent |
| 產(chǎn)品應(yīng)用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 0.3071 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Brdu |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis Subcellular Location: Nuclear. Proliferation Marker BrdU（溴化脫氧尿嘧啶核苷）可以在體內(nèi)和體外摻入到處于S期的細(xì)胞所合成的DNA鏈中。 此抗體可以與摻入到任何種屬細(xì)胞中的BrdU反應(yīng)，與碘脫氧尿 嘧啶有交叉反應(yīng)，標(biāo)記摻有BrdU的S期細(xì)胞，主要用于研究各種 不同的因素對(duì)正常/腫瘤組織的細(xì)胞增殖及動(dòng)力學(xué)的研究。 用FITC標(biāo)記的抗BrdU IgG可用于流式細(xì)胞術(shù)定量檢測(cè)增值細(xì)胞。 |
