| 產品編號 | bs-0191R |
| 英文名稱 | HSP60 Rabbit pAb |
| 中文名稱 | 熱休克蛋白-60/groEL抗體 |
| 別 名 | HSP 60; HSP-60; 60 kDa chaperonin; 60 kDa heat shock protein mitochondrial; 60kDa; cb863; Chaperonin 60; Chaperonin; Chaperonin, 60-KD; CPN 60; CPN60; fa04a05; fb22d10; fi27b05; GROEL; GroEL Homolog; GroEL, E. coli, homolog of; Heat shock 60kD protein 1(c |
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Specific References (9) | bs-0191R has been referenced in 9 publications.
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| 研究領域 | 腫瘤 免疫學 信號轉導 轉錄調節因子 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HSP60: 501-573/573 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. Function: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Subunit: Interacts with HRAS (By similarity). Interacts with HBV protein X and HTLV-1 protein p40tax. Interacts with ATAD3A. Subcellular Location: Mitochondrion matrix. DISEASE: Spastic paraplegia autosomal dominant 13 (SPG13) [MIM:605280]: A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Note=The disease is caused by mutations affecting the gene represented in this entry. Leukodystrophy, hypomyelinating, 4 (HLD4) [MIM:612233]: A severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurs within the first two decades of life. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the chaperonin (HSP60) family. SWISS: P10809 Gene ID: 3329 Database links: Entrez Gene: 3329 Human Entrez Gene: 15510 Mouse Omim: 118190 Human SwissProt: P86206 Hamster SwissProt: P10809 Human SwissProt: P63038 Mouse Unigene: 595053 Human Unigene: 727543 Human Unigene: 1777 Mouse Unigene: 102058 Rat 信號傳導（Signaling Intermediates） HSP60是一類進化上高度保守的蛋白質家族.生理狀態時協助多肽或蛋白質的正確轉位、折疊和裝配,起"分子伴侶"的作用;在應激狀態下,HSP60過表達或異位表達,作為一種自身抗原被免疫系統識別,誘發機體的保護性免疫應答,也可作為一種信號分子,在信號轉導中發揮作用. 該蛋白與腫瘤的發生、增殖及分化有關，主要用于舌鱗癌、結腸癌、胰腺癌和乳腺癌的研究，近年來研究證實HSP60在自身免疫性疾病、傳染病、動脈粥樣硬化及慢性感染的發病中均發揮重要的作用。 HSP-60熱休克蛋白-蛋白質折疊過程的引導因子 分子伴侶是一種引導蛋白質正確折疊的蛋白質。當蛋白質折疊時，它們能保護蛋白質分子免受其它蛋白質的干擾。很多分子伴侶屬于熱休克蛋白（例如HSP-60），它們在細胞受熱時大量合成。熱激可導致蛋白質穩定性降低，增加錯誤折疊的幾率，因此在受到熱刺激時，細胞中的蛋白質需要更多熱休克蛋白的幫助。 HSP-60是一種典型的分子伴侶，它可以為正在折疊的蛋白質提供一個附著環境，從而起到保護折疊過程的作用。 |
| 產品圖片 |
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Adrenal gland (Mouse) Lysate at 40 ug
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 4: Adrenal gland (Rat) Lysate at 40 ug
Lane 5: Jurkat (Human) Cell Lysate at 30 ug
Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 ug
Lane 7: HepG2 (Human) Cell Lysate at 30 ug
Lane 8: Hela (Human) Cell Lysate at 30 ug
Lane 9: K562 (Human) Cell Lysate at 30 ug
Primary: Anti-HSP60 (bs-0191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP60) Polyclonal Antibody, Unconjugated (bs-0191R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated(bs-0191R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated(bs-0191R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP60) polyclonal Antibody, Unconjugated (bs-0191R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
