| 產品編號 | bs-1049R |
| 英文名稱 | CHRNA7 Rabbit pAb |
| 中文名稱 | 煙堿型乙酰膽堿受體α7抗體 |
| 別 名 | CHRFAM7A; ACHA7_HUMAN; cholinergic receptor, nicotinic, alpha 7; Neuronal acetylcholine receptor subunit alpha-7; ACHR ALPHA 7; AChR alpha 7 Receptor; Acra7; ALPHA-7NACHR; ALPHA7; ALPHA7 NICOTINIC ACETYLCHOLINE RECEPTOR; alpha7 nicr; BTX; CHRNA7; CHRNA7-2 nicotinic α7; nicotinicα7; nicotinic α 7; nicotinic α-7; nicotinic-α7. |
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Specific References (9) | bs-1049R has been referenced in 9 publications.
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| 研究領域 | 細胞生物 神經生物學 信號轉導 細胞凋亡 通道蛋白 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Chicken) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CHRNA7: 441-502/502 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The Nicotinic Acetylcholine Receptors are members of a superfamily of ligand gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be hetero pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C terminal extracellular region. The Nicotinic Acetylcholine Receptor alpha 7 forms a homo oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane. Function: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. Subunit: Homopentamer. Interacts with RIC3; which is required for proper folding and assembly. Subcellular Location: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily. SWISS: Q8IUZ4 Gene ID: 1139 Database links: Entrez Gene: 1139 Human Entrez Gene: 374001 Chicken Entrez Gene: 11441 Mouse Omim: 118511 Human SwissProt: P22770 Chicken SwissProt: P36544 Human SwissProt: Q8IUZ4 Human SwissProt: P49582 Mouse Unigene: 88 Cow Unigene: 511772 Human Unigene: 113464 Mouse Unigene: 9698 Rat |
| 產品圖片 |
Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Adrenal glands (Rat) Lysate at 40 ug
Lane 4: Adrenal glands (Mouse) Lysate at 40 ug
Lane 5: Placenta (Mouse) Lysate at 40 ug
Lane 6: Lung (Mouse) Lysate at 40 ug
Lane 7: Testis (Rat) Lysate at 40 ug
Lane 8: HepG2 (Human) Cell Lysate at 30 ug
Lane 9: SH-SY5Y (Human) Cell Lysate at 30 ug
Primary: Anti-CHRNA7 (bs-1049R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56’50 kD
Observed band size: 48 kD
Sample:
Lane 1: Human SH-SY5Y cell Lysates
Lane 2: Human U-87 MG cell Lysates
Lane 3: Human U251 cell Lysates
Primary: Anti-CHRNA7 (bs-1049R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kDa
Observed band size: 55kDa
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated(bs-1049R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CHRNA7) Polyclonal Antibody, Unconjugated (bs-1049R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated(bs-1049R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
