| 產品編號 | bs-1119R |
| 英文名稱 | PAF Rabbit pAb |
| 中文名稱 | 血小板活化因子抗體 |
| 別 名 | platelet-activating factor; 1-Hexadecyl-2-acetyl-sn-glycero-3-phosphocholine; 1-O-Palmityl-2-acetyl-sn-glycero-3-phosphocholine monohydrate; 2-Acetyl-1-hexadecyl-sn-glycero-3-phosphocholine; 3-sn-Phosphatidylcholine; 1-hexadecyl-2-acetyl, PAF(C16. |
| 產品類型 | 小分子抗體 |
| 研究領域 | 藥物及化合物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: PAF) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 0.524 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated PAF |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Platelet-activating factor, a compound that reduces inflammation by increasing permeability of blood vessels and contracting various involuntary muscles such as those in airways. It is a phospholipid with a wide range of proinflammatory actions, is immediately degraded and inactivated in vivo by PAF acetylhydrolase. 血小板活化因子（platelet-activating factor，PAF）是一種內源性具有生物活性的磷脂。由中性粒細胞、血小板、肥大細胞、內皮細胞和巨噬細胞產生。除早期發現它有強烈引起血小板聚集和脫顆粒作用外，近年來的研究發現它還具有廣泛的生物活性，作為一種獨特的細胞因子參與體內多種生理、病理過程，包括激活中性粒細胞，使其聚集、趨化、釋放氧自由基和白三烯，抑制T細胞增殖，刺激B細胞合成IgG和IgE，抑制心肌收縮力，增加血管通透性，引起體循環血壓下降和肺動脈高壓等。由于它具有廣泛的高強度的生物活性，故PAF被認為是與過敏反應有關的關鍵性介質之一。 |
| 產品圖片 |
Tissue/cell: rat colon tissue;4% Paraformaldehyde-fixed and paraffin-embedded
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min
Incubation: Anti-PAF Polyclonal Antibody, Unconjugated(bs-1119R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
