| 產品編號 | bsm-33128M |
| 英文名稱 | Plant actin Mouse mAb, Loading Control |
| 中文名稱 | 植物肌動蛋白（內參）單克隆抗體 |
| 別 名 | Plant-actin; beta Actin; 植物內參; 植物肌動蛋白內參; |
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Specific References (1) | bsm-33128M has been referenced in 1 publications.
[IF=6.064] Jianan Hu. et al. Identification of Key Residues Essential for the Activation of Plant Immunity by Subtilisin From Bacillus velezensis LJ02.. FRONT MICROBIOL. 2022 Aug;13:869596-869596 WB ; Nicotiana benthamiana.
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| 產品類型 | 內參抗體 植物抗體 |
| 研究領域 | 細胞生物 發(fā)育生物學 植物 細胞骨架 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 4F5 |
| 交叉反應 | Various Plants |
| 產品應用 | WB=1:2000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Synthetic MAP peptide derived from human beta-Actin |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Antibodies against plant Actin are useful as loading controls for Western Blotting Database links: SwissProt: P0CJ46 ARATH |
| 產品圖片 |
Sample:
Lane 1: Waxy corn (Plant) Lysate at 40 ug
Lane 2: Brassica napus (Plant) Lysate at 40 ug
Primary: Anti-Plant actin (bsm-33128M) at 1/1000 dilution
Secondary: IRDye800CW Goat Ant-Mouse IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 44 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
