| 產(chǎn)品編號(hào) | bsm-33004M |
| 英文名稱 | His tag Mouse mAb |
| 中文名稱 | 聚組氨酸抗體標(biāo)簽單克隆抗體 |
| 別 名 | HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine. |
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Specific References (1) | bsm-33004M has been referenced in 1 publications.
[IF=4.961] Rong Yue. et al. Study of the Effects of Several SARS-CoV-2 Structural Proteins on Antiviral Immunity. VACCINES-BASEL. 2023 Mar;11(3):524 WB ; Human.
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| 產(chǎn)品類型 | 標(biāo)簽抗體 |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | 6000000 |
| 交叉反應(yīng) | Recombinant protein |
| 產(chǎn)品應(yīng)用 | WB=1:5000-50000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated HHHHHH peptide |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. 蛋白質(zhì)純化的新途徑─應(yīng)用His-Tag抗體檢測(cè)His-Tag融合蛋白 1） 6xHis與不同的Sepharose純化填料結(jié)合（捆綁），可不受構(gòu)象的影響，在天然或變形的條件下可進(jìn)行進(jìn)一步純化。 2） 可在溫和的洗脫液環(huán)境中，其結(jié)合、洗滌及洗脫步驟不會(huì)對(duì)蛋白結(jié)構(gòu)產(chǎn)生影響。 3） 6xHis標(biāo)簽比普通（Tag）要小得多，經(jīng)純化后的蛋白可直接進(jìn)行下游操作?？捎糜谌魏伪磉_(dá)系統(tǒng)（包括： 細(xì)菌，桿狀病毒和哺乳動(dòng)物） 4） 6xHis標(biāo)簽在生理溶液pH下不帶電荷，其標(biāo)簽不會(huì)干涉重組蛋白的結(jié)構(gòu)和功能， 6xHis不會(huì)影響蛋白分泌。 5） 6xHis標(biāo)簽免疫原性差，重組蛋白可以不必除去標(biāo)簽而直接作為抗原進(jìn)行免疫。 |
| 產(chǎn)品圖片 |
Sample: Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Primary: Anti-His tag (bsm-33004M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD
50 ng Recombinant GFP protein (bs-33009P) per lane probed with His tag monoclonal antibody respectively, unconjugated (bsm-33004M) at 1:5000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Sample:
His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4 ug
Primary: Anti-His tag (bsm-33004M) at 1/2000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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