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CD8 Rabbit pAb (bs-23448R)  
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50ul/1180.00元
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產品編號 bs-23448R
英文名稱 CD8 Rabbit pAb
中文名稱 CD8抗體
別    名 CD8 antigen, alpha polypeptide(p32); CD8a; CD8a antigen; CD8a molecule; CD8A_MOUSE; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differen  
研究領域 細胞生物  免疫學  干細胞  細胞表面分子  淋巴細胞  t-淋巴細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse
產品應用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 27 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse CD8: 51-150/247 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011].

Function:
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subunit:
In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
P01731

Gene ID:
12525

Database links:

Entrez Gene: 925 Human

Entrez Gene: 926 Human

Entrez Gene: 12525 Mouse

Entrez Gene: 12526 Mouse

Omim: 186910 Human

SwissProt: P01732 Human

SwissProt: P10966 Human

SwissProt: P01731 Mouse

SwissProt: P10300 Mouse

Unigene: 405667 Human

Unigene: 85258 Human

Unigene: 1858 Mouse

Unigene: 333148 Mouse



產品圖片
Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-CD8 (bs-23448R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD
Sample: Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti-CD8 (bs-23448R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD
Sample: Blood (Mouse) Lysate at 40 ug Primary: Anti- CD8 (bs-23448R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 32kD
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